Voxengo Elephant 3 Serial Dilutions

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Voxengo Elephant 3 Serial Dilutions Average ratng: 4,2/5 3602 reviews

Advantages of 'Serial Dilutions'This section is not a recipe for your experiment. It explains someprinciples for designing dilutions that give optimal results. Onceyou understand these principles, you will be better able to designthe dilutions you need for each specific case.Often in experimental work, you need to cover a range ofconcentrations, so you need to make a bunch of differentdilutions. For example, you need to do such dilutions of thestandard IgG to make the standard curve in ELISA, and then againfor the unknown samples in ELISA.You might think it would be good to dilute 1/2, 1/3, 1/10, 1/100.These seem like nice numbers. There are two problems with this series ofdilutions. The dilutions are unnecessarily complicated to make.

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You need to do a differentcalculation, and measure different volumes, for each one. It takes a longtime, and it is too easy to make a mistake. The dilutions cover the range from 1/2 to 1/100 unevenly.In fact, the 1/2 vs. 1/3 dilutions differ by only 1.5-fold in concentration,while the 1/10 vs.

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1/100 dilutions differ by ten-fold. If you are going tomeasure results for four dilutions, it is a waste of time and materialsto make two of them almost the same. And what if the half-maximal signaloccurs between 1/10 and 1/100?

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